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Item #:
124802616
Liquid Chromatography Column C18 4.6 150mm 00F-4252-E0
Item #:
124802616
AED 1048
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Chromatographic columns provide high-resolution separation and are suitable for the analysis of complex samples.
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What Stands Out
Superior Resolution
The C18 column provides exceptional separation efficiency, ensuring precise analysis of samples, making it ideal for complex mixtures and satisfying stringent quality control standards.
Robust Durability
Built with high-quality materials, this column withstands various conditions, offering consistent performance over time and reducing the need for frequent replacements in the laboratory setup.
Versatile Applications
Designed for a wide range of applications, this column excels in pharmaceutical, environmental, and food analysis, catering to diverse user needs while enhancing laboratory productivity.
Product Details
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Research Laboratories
Ideal for researchers needing reliable separation in biochemical and analytical studies using liquid chromatography techniques.
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Pharmaceutical Companies
Beneficial for pharmaceutical testing and analysis, ensuring accurate separation of complex mixtures in drug formulations.
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Environmental Testing
Useful for environmental scientists analyzing pollutants or contaminants in water and soil samples.
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Beginner Experimenters
Not suitable for novices who may not fully understand the complexities of liquid chromatography setups.
Product Description
Liquid Chromatography Column C18 4.6 150mm 00F-4252-E0
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Customer Questions & Answers
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Question:
What is a Liquid Chromatography Column C18 4.6 × 150mm used for?
Answer: The Liquid Chromatography Column C18 4.6 × 150mm is primarily used for separating and analyzing different compounds in a sample through reversed-phase liquid chromatography. This column is ideal for applications in pharmaceuticals, environmental testing, and biochemical research. The C18 packing material provides a non-polar surface, enabling efficient retention of hydrophobic molecules. For instance, if you are purifying a pharmaceutical compound, this column can help isolate the target analytes from other substances in the mixture. -
Question:
What types of samples can be analyzed using the C18 column?
Answer: The C18 column can effectively analyze a wide range of sample types, including organic compounds, pharmaceuticals, and biological samples. It excels in separating non-polar to moderately polar compounds due to its hydrophobic nature. For example, you can use this column to analyze drug formulations or metabolites in biological fluids, ensuring accurate and precise separation of components for quality control or research purposes. -
Question:
How do I prepare my sample for use with the C18 column?
Answer: Sample preparation for the C18 column typically involves dissolving the analyte in a suitable solvent, often a mobile phase compatible with the column's specifications. It is essential to filter the sample to remove particulates, which could clog the column. This preparation enhances the reproducibility and efficiency of the chromatographic process. For example, if analyzing a sample containing proteins, you might use centrifugation and filtration before injection into the column. -
Question:
What is the maximum pressure tolerance of the C18 4.6 × 150mm column?
Answer: The C18 4.6 × 150mm column typically has a maximum pressure tolerance ranging from 4000 to 6000 psi, depending on the manufacturer. Exceeding this pressure could damage the column and affect its performance. To maintain optimal results, it is crucial to monitor the system pressure during operation. For applications requiring high-resolution separation, staying within these limits ensures longer column life and effective separation of analytes. -
Question:
Can the C18 column be used for gradient elution?
Answer: Yes, the C18 column is well-suited for gradient elution techniques, which allow for enhanced separation of complex mixtures. Gradient elution involves varying the mobile phase composition over time, helping to resolve closely eluting compounds. For example, in drug purity analysis, a gradient can effectively separate contaminants or degradation products from the active pharmaceutical ingredient, improving the accuracy of your results. -
Question:
How do I clean or maintain the C18 column?
Answer: Cleaning and maintaining the C18 column involves flushing it with a solvent that is compatible with the stationary phase. Regular maintenance helps prevent contamination and blockages, ensuring consistent performance. It is advisable to backflush the column or run a cleaning solution after analyzing particularly dirty samples. For optimal results, perform routine checks on the baseline noise and resolution to identify any decline in performance and act accordingly. -
Question:
Is the C18 column compatible with HPLC and UHPLC systems?
Answer: The C18 4.6 × 150mm column is compatible with both HPLC and UHPLC systems, making it versatile for different applications. While HPLC uses lower pressures and larger particle sizes, UHPLC allows for higher pressures and finer particles, providing better resolution and speed. If you have access to a UHPLC system, using the C18 column can significantly enhance your separations. This adaptability makes it suitable for laboratories using various chromatographic technologies. -
Question:
What are some common applications of the C18 column?
Answer: Common applications of the C18 column include analysis of pharmaceuticals, natural products, and food safety testing. It is frequently used for quality control in pharmaceutical manufacturing to ensure that active ingredients are correctly formulated. Researchers also rely on the C18 column for hormone analysis or analyzing environmental pollutants, demonstrating its broad applicability across different fields. -
Question:
What is the lifespan of a Liquid Chromatography C18 column?
Answer: The lifespan of a Liquid Chromatography C18 column can vary greatly depending on the type of samples analyzed, the mobile phase used, and the operating conditions. Generally, with proper maintenance and care, a C18 column can last from a few hundred to several thousand injections. Regularly monitoring performance and performing preventive maintenance can help maximize its lifespan, allowing researchers to maintain consistent results over time. -
Question:
Where can I buy Liquid Chromatography Column C18 4.6 × 150mm 00F-4252-E0?
Answer: You can purchase the Liquid Chromatography Column C18 4.6 × 150mm 00F-4252-E0 at Ubuy. They offer a comprehensive range of laboratory supplies, including high-quality chromatography columns suitable for various analytical applications. By shopping at Ubuy, you can ensure you’re getting a reliable product along with excellent customer service.
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Features & Benefits
- High-resolution separation for complex samples.
- Ideal for pharmaceutical and analytical applications.
- Manufactured for repeatability and reproducibility.
- Supports a variety of separation modes.
- Designed to withstand high flow rates.
- Perfect for high throughput analyses and rapid separations.
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