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Item #:
200973946
UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal 100ml
Item #:
200973946
AED 246
Price Details
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What Stands Out
Ready-to-Use Solution
This 1-component substrate eliminates the need for complex mixing, providing a hassle-free experience for users while ensuring consistent results in various applications, including immunoassays and enzyme-linked assays.
Wide Compatibility
Designed to work universally with multiple HRP (Horseradish Peroxidase) detection systems, this substrate enhances versatility, making it suitable for a broad range of experimental setups and techniques.
Enhanced Sensitivity
Offers superior sensitivity and signal amplification, enabling the detection of low-abundance targets effectively, which is critical for researchers aiming for reliable and precise data in their assays.
Product Details
| Item Weight | 0.11 lbs (50 grams) |
Who Should Buy?
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Research Laboratories
Ideal for academic and corporate laboratories conducting enzyme-linked immunosorbent assays (ELISAs) with sensitive detection requirements.
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Quality Control
Suitable for industries requiring precise and sensitive quantitative measurements in product testing and quality assurance.
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Clinical Diagnostics
Beneficial for clinical settings performing diagnostic tests that rely on HRP enzyme-substrate reactions for accuracy.
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Casual Users
Not suitable for hobbyists or individuals without specialized knowledge in laboratory techniques for enzyme reactions.
Product Description
UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal 100ml
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is UltraSensitive TMB HRP Aqueous Substrate used for?
Answer: UltraSensitive TMB HRP Aqueous Substrate is a high-sensitivity, one-component substrate designed for use in enzyme-linked immunosorbent assays (ELISA) and other biochemical assays. It is utilized to detect horseradish peroxidase (HRP) enzyme activity, providing a chromogenic reaction that produces a color change. This substrate is particularly useful for applications requiring highly sensitive detection, making it ideal for researchers looking to optimize assay results in clinical diagnostics and research laboratories. -
Question:
What are the key features of this substrate?
Answer: The UltraSensitive TMB HRP Aqueous Substrate boasts several key features, including high sensitivity and stability, which ensure consistent and reliable results. Its convenient one-component formulation simplifies preparation and reduces the chance of error during use. Moreover, the substrate produces a blue color upon reaction, which can be quantitatively measured with a spectrophotometer. This substrate is suited for applications in both academic and industrial labs that require precision and reproducibility in enzyme assays. -
Question:
How should I store the UltraSensitive TMB HRP Aqueous Substrate?
Answer: To maintain the integrity and prolong the shelf life of UltraSensitive TMB HRP Aqueous Substrate, it should be stored in a cool, dark place, preferably at refrigeration temperatures. Avoid exposure to prolonged light and heat, as these conditions can adversely affect the substrate's activity. Proper storage is crucial for ensuring optimal performance, especially when conducting sensitive experiments where accurate measurements are necessary. -
Question:
Can the UltraSensitive TMB HRP Aqueous Substrate be used in both ELISA and Western Blot applications?
Answer: Yes, UltraSensitive TMB HRP Aqueous Substrate is versatile and can be effectively utilized in both ELISA and Western Blot applications. For ELISA, it enhances the detection of target antigens due to its high sensitivity. In Western Blotting, it aids in the visualization of HRP-conjugated antibodies, which is essential for identifying specific proteins. This dual application capability makes it a valuable tool for researchers working in various fields of bioanalysis. -
Question:
What is the lifespan of the color developed with this substrate?
Answer: The color developed with UltraSensitive TMB HRP Aqueous Substrate typically remains stable for a period that allows for thorough analysis, often several hours, depending on the assay conditions. It is advisable to measure the absorbance soon after the reaction reaches its peak to ensure the most accurate results. Conducting readings promptly ensures that you capture the maximum intensity of the reaction, providing reliable data for your analysis. -
Question:
Is this substrate compatible with different assay buffers?
Answer: UltraSensitive TMB HRP Aqueous Substrate is generally compatible with a variety of assay buffers, including phosphate-buffered saline (PBS) and Tris-buffered saline (TBS), often used in enzyme assays. However, it is important to optimize buffer conditions for your specific application to achieve the best performance. Validation of the substrate's activity within your assay buffer is recommended to ensure that the enzymatic reaction proceeds as expected. -
Question:
How do I prepare the UltraSensitive TMB HRP Aqueous Substrate for use?
Answer: One of the advantages of UltraSensitive TMB HRP Aqueous Substrate is that it comes in a ready-to-use, one-component format. Simply pipette the required volume into your assay setup without the need for any additional preparation steps. This simplifies the workflow in your lab, allowing you to focus on your experimental design rather than complex preparation protocols, reducing the potential for variability in results. -
Question:
What are the safety precautions to take when handling this substrate?
Answer: When handling UltraSensitive TMB HRP Aqueous Substrate, it's important to adhere to standard laboratory safety practices. Wearing gloves and safety goggles is advised to protect your skin and eyes from direct contact, as well as to minimize exposure to any potentially hazardous materials. Additionally, ensure that you're working in a well-ventilated area. Following these precautions helps maintain a safe research environment while using chemical reagents. -
Question:
What is the significance of using a one-component substrate in assays?
Answer: Using a one-component substrate like UltraSensitive TMB HRP Aqueous Substrate provides significant advantages in assay simplicity and consistency. It eliminates the need for mixing multiple components, reducing the risk of preparation errors and variability in results. This simplification is beneficial for high-throughput applications, where consistency and reliability are paramount. Researchers can focus more on their experimental designs rather than procedural complexities, enhancing overall efficiency. -
Question:
Where can I buy UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml) in UAE?
Answer: You can purchase UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml) from Ubuy, which offers a convenient platform for obtaining this product in UAE. Ubuy provides a streamlined shopping experience, ensuring that you can get the necessary reagents quickly and efficiently, along with detailed product information and customer support.
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AED 246
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Features & Benefits
- Detects horseradish peroxidase (HRP) activity.
- Produces a blue color that turns yellow with Stop 450 Solution.
- Ready-to-use formulation for convenience.
- Non-carcinogenic and free from toxic substances.
- Stable for 2-3 years when stored at 4°C.
- Shipped at room temperature for easy handling.
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