What is Chaps Lysis Buffer used for?

Chaps Lysis Buffer is primarily utilized for lysing cells in biochemical applications. It aids in the extraction of proteins while maintaining their functionality. This is critical in various research settings, such as proteomics or molecular biology, where high-quality protein samples are needed for further analysis, such as Western blotting or enzyme assays.

How do I prepare samples using Chaps Lysis Buffer?

To prepare your samples, you should first dilute the Chaps Lysis Buffer according to your protocol. Typically, a 1:1 ratio with your sample is recommended. Once mixed, incubate the solution on ice for approximately 30 minutes. This method ensures optimal cell lysis and preservation of protein structures, making it valuable for sensitive assays or analyses.

Is Chaps Lysis Buffer compatible with all protein assays?

Chaps Lysis Buffer is compatible with many protein assays, including SDS-PAGE and ELISA, but it’s essential to validate its suitability for your specific assay. The detergent properties can influence protein solubility and detection, so performing preliminary tests is advisable to ensure consistent results.

Can I store Chaps Lysis Buffer after opening?

Yes, after opening, it’s best to store Chaps Lysis Buffer in a cool, dry place and protect it from light. Proper storage extends its usability. However, it’s important to label the container with the date of opening and to check for any precipitate or changes in clarity before each use, ensuring you achieve the best results in your experiments.

What are the components of Chaps Lysis Buffer?

Chaps Lysis Buffer typically contains CHAPS, a zwitterionic detergent, along with salts and buffering agents that create a stable environment for protein extraction. The components are chosen carefully to ensure effective cell lysis while preventing protein denaturation, making it an essential tool in the research labs focused on maintaining protein integrity.

Can I use Chaps Lysis Buffer for both plant and animal tissues?

Yes, Chaps Lysis Buffer is versatile and can be used for lysing both plant and animal tissues. The buffer’s formulation allows for effective solubilization of proteins from various biological sources, thus facilitating analysis in cross-species research, such as comparative proteomics and metabolic studies.

How much Chaps Lysis Buffer should I use per sample?

The amount of Chaps Lysis Buffer you should use depends on the sample type and its cellular concentration. A common practice is to start with a ratio of 1 mL buffer per 100 mg of tissue. Adjust this as needed based on efficiency observed in your specific application, particularly when dealing with high-density cell cultures or tissue samples.

Is Chaps Lysis Buffer suitable for membrane protein extraction?

Yes, Chaps Lysis Buffer is particularly suitable for membrane protein extraction due to its amphipathic nature. It helps solubilize proteins without extensively disrupting protein-protein interactions. This makes it a preferred choice for studies focusing on membrane-associated proteins, where maintaining structural integrity is vital for functional assays.

Are there any safety precautions I should take when using Chaps Lysis Buffer?

When using Chaps Lysis Buffer, it is advisable to wear gloves, safety goggles, and lab coats to minimize skin and eye contact. While it is generally considered safe for laboratory use, always consult the safety data sheet (SDS) for detailed hazards and handling information, ensuring a safe working environment.

Where can I buy Chaps Lysis Buffer (2X)- Each (25ml) in UAE?

You can buy Chaps Lysis Buffer (2X)- Each (25ml) at Ubuy. Ubuy specializes in providing a wide range of scientific supplies and reagents, making it easy to find and purchase quality products for your research needs.

How to Shop Chaps Lysis Buffer (2X)- Each (25ml) Online From Ubuy?

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Is Chaps Lysis Buffer (2X)- Each (25ml) Available to Shop Online in UAE?

Yes, at Ubuy UAE this product is available for you to shop at a reasonable price. The Chaps Lysis Buffer (2X)- Each (25ml) is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are essential for protein research and molecular biology.

eCon Lab Supply

Item #: 211186853

Chaps Lysis Buffer (2X)- Each (25ml)

Item #: 211186853

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What Stands Out

Concentrated Formula

The 2X concentration ensures effective lysis of cells, maximizing the yield of proteins and nucleic acids, making it ideal for various laboratory applications.

Versatile Use

Suitable for a wide range of biological samples, the buffer effectively aids in protein extraction, DNA/RNA isolation, and other critical research processes.

Ease of Use

Designed for straightforward application, this buffer reduces preparation time and allows researchers to focus on their experiments with minimal hassle.

Product Details

Item Weight	4 ounces (113.4 grams)
Date First Available	March 9, 2022
Manufacturer	Ciro Mfg
Container Type	Bottle
Item Form	Liquid
Brand Name	eCon Lab Supply
Unit Count	1 Count
Included Components	Bottle
Part Number	23.105.123
Volume	25 ml

Who Should Buy?

Molecular Biologists
Ideal for genetic analysis and protein extraction procedures in molecular biology research labs.
Cell Biologists
Useful for lysing cells to extract proteins for various cellular assays and experiments.
Researchers
Beneficial for laboratory researchers needing reliable and effective lysis buffer for sample preparation.

General Consumers
Not suitable for individual use outside of scientific research or without proper laboratory equipment.

Product Description

Chaps Lysis Buffer (2X)- Each (25ml)

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Customer Questions & Answers

Question: How to Shop Chaps Lysis Buffer (2X)- Each (25ml) Online From Ubuy?

Answer: It’s easy to shop Chaps Lysis Buffer (2X)- Each (25ml) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Chaps Lysis Buffer (2X)- Each (25ml) Available to Shop Online in UAE?

Answer: Yes, at Ubuy UAE this product is available for you to shop at a reasonable price. The Chaps Lysis Buffer (2X)- Each (25ml) is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply Buffers Editorial Review

Chaps Lysis Buffer (2X)- Each (25ml) is an efficient liquid solution that's particularly beneficial for cellular disruption in various laboratory applications. Manufactured by Ciro Mfg and distributed by eCon Lab Supply, this 25 ml bottle is carefully designed for ease of use. Users have noted its effectiveness in improving cell lysis rates, making it a go-to choice for researchers in need of reliable results. The product has been available since March 9, 2022, providing a stable option for consistent lab work without complications related to dosing or delivery. It's an ideal solution in your lab toolkit, well-received by its community.

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Pros

Suitable for effective cellular disruption
Compact 25 ml bottle for lab use
Lightweight and easy to handle
Simple to use for various experiments
Reliable performance in cell lysis applications

Cons

Contains a smaller volume for frequent usage

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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AED 366

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Features & Benefits

Comprehensive range of biological buffers for research and diagnostics.
Essential for protein research, molecular biology, and characterization.
Manufactured in a USA facility certified under ISO 9001 and 13485.
Produced following stringent Good Manufacturing Practices.
Quality control tests conducted under certified 14644 clean room conditions.
Designed specifically for cell lysis and protein extraction.

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Chaps Lysis Buffer (2X)- Each (25ml)

AED 366

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What Stands Out

Product Details

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Product Description

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eCon Lab Supply Buffers Editorial Review

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Product Price History

Important information

AED 366

Features & Benefits

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